Figure 2. Increased levels of oxidized proteins and DNA in the RPE of aged Atg5^ΔRPE mice. Frozen sections from 8-month-old wild-type (+/+; A, C, E) and Atg5^ΔRPE (Atg5−/−; B, D, F) mice were stained with antibodies (green signal) against 8-hydroxy-2’-deoxyguanosine (8-OHdG; A and B), 3-nitrotyrosine (C and D), or advanced glycation end products (AGEs; E and F). Note the bright green punctate staining in the RPE of the Atg5^ΔRPE mice. The nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; blue signal).