Figure 2 of Hollborn, Mol Vis 2017; 23:116-130.


Figure 2. Involvement of transcription factor activities in the osmotic expression of the NFAT5 gene in RPE cells. The level of NFAT5 mRNA was determined with real-time reverse transcriptase (RT)-PCR analysis in cells cultured 6 h in iso- (control) and hyperosmotic (+100 mM NaCl) media. The following blocking agents were tested: a hypoxia-inducible transcription factor (HIF) inhibitor (HIF-Inh; 5 µM), the STAT3 inhibitor Stattic (1 µM), and the NF-κB inhibitor caffeic acid phenethyl ester (CAPE; 5 µM). Means ± standard error of the mean (SEM) of 5–7 independent experiments using cell lines from different donors. Significant difference versus unstimulated control: *p<0.05. Significant difference versus NaCl control: ●p<0.05.