Figure 2. Effects of bortezomib on migration of APRE-19. A: In vitro wound-healing assay revealed that bortezomib, in comparison with vehicle, significantly inhibited wound healing in RPE monolayers. B: Quantification of the remaining wound area. Bortezomib inhibited wound healing after 24 h. ***p<0.001 with an independent t test. C: Transwell migration assays revealed that bortezomib decreased RPE migration. D: Quantification of migrated RPE cells revealed a statistically significant decrease in migration by treatment with 20 nM bortezomib compared to that of control cells. ***p<0.001 with an independent t test, n=3, standard error. E: Filamentous actin staining revealed that bortezomib decreased the number of stress fibers and lamellipodia involved in cell migration. F: Western blot analysis of RAC1 and RHOG revealed that bortezomib downregulated RAC1/RHOG expression. G: Immunocytochemical analysis confirmed that bortezomib downregulated RAC1/RHOG expression.