The cortical (C), nuclear (N) or posterior subcapsular (P) opacity of the cataract was classified with the Lens Opacities Classification System III (LOCS III), with an increasing cataract severity from Group I to Group IV. Group I: C2-3N1-2; Group II, C3-4N2-3; Group III, C4-5N3-4; Group IV, C4-5N4-5; Group V, posterior subcapsular senile cataract (P2-P4, with C<2 and N<2); Group VI, cataract with myopia; Group VII, cataract with diabetes; and the healthy Group, healthy tissue samples. In each group, three independent experiments were performed. The data are the means ± standard deviation (SD). No significant differences among all eight groups were seen, by one-way ANOVA and least significant difference (LSD) tests (p>0.05). Healthy samples were used as normal controls. To access the data, click or select the words “Appendix 2”