Figure 2. Example of quantification of NETs in different specimens. Row 1: none, Row 2: +, Row 3: ++, Row 4: +++, respectively, representing the number of DNA-releasing neutrophils as +: <10%, ++: 10% to 30%, and +++: 31% to 50%. The negative control was taken from a negative field of a positive sample. The structure of the multilobular nuclei of neutrophils can be seen in the left top image indicated with an arrow. Morphology of fungal hyphae was also determined in the Row 4 images (arrowhead). DNA (left panel), elastase (middle panel), Merge (right panel). Bar: 100 μm.