Figure 6. Impact of RF/6A cell tube formation by TF expression in ARPE-19 cells. A: Representative photographs of tube formation in each group (40X magnification). B: The average number of closed networks of tubes per field. Silence of tissue factor (TF) greatly reduced the tube formation of cocultured RF/6A cells (*p<0.01, TF siRNA versus Control). Overexpression of TF greatly increased the tube formation of cocultured RF/6A cells (*p<0.01, pEGFP-N3-hTF versus Control), and this effect was significantly reduced with the Wnt inhibitor (#p<0.01, pEGFP-N3-hTF versus pEGFP-N3-hTF+IWR-1-endo). Bar = 100 μm.