Table 1. Cell and compartmental specific primary antibodies.
| Primary antigen |
Abbreviation |
Immunogen |
Host |
Source |
Dilution |
References |
| Glycolysis |
|
|
|
|
|
| Hexokinase 1 |
HK1 |
Human HK1 |
Rabbit |
Cell Signaling |
1:500 |
89 |
| Hexokinase 2 |
HK2 |
Human AA 341 size16-20 AA |
Rabbit |
Cell Signaling |
1:200 |
88-90 |
| Phosphofructokinase L1 |
PFK-L1 |
Human Synthetic Pep AA 669-699 C-terminus |
Rabbit |
Abgent |
1:200 |
|
| Pyruvate kinase M1 |
PK-M1 |
Human Synthetic Peptide AA 398-416 |
Rabbit |
Sigma |
1:500 |
91 |
| Pyruvate kinase M2 |
PK-M2 |
Human AA 380-440 |
Rabbit |
Abcam |
1:500 |
92 |
| Pyruvate kinase M2 |
PK-M2 |
Human PK-M2 full length |
Rabbit |
Cell Signaling |
1:500 |
30, 55 |
| Pan-lactate dehydrogenase |
pan-LDH |
Human LDH |
Rabbit |
Santa Cruz |
1:500 |
|
| Lactate dehydrogenase 5 |
LDH5 |
Human LDH AA 203-232 C-terminus |
Rabbit |
Abgent |
1:500 |
|
| TCA cycle and OXPHOS |
|
|
|
|
| Oxoglutarate (α-ketoglutarate) dehydrogenase |
OGDH |
Human OGDH AA 705-1000 |
Rabbit |
Thermo Scientific |
1:500 |
|
| Glutaminase |
GLS |
Human GLS AA 580-670 |
Mouse |
Abcam |
1:500 |
93-95 |
| Glutamate dehydrogenase 1 |
GDH 1 |
Recombinant GDH1 AA 1-265 |
Mouse |
Novus Biologicals |
1:500 |
|
| GABA transaminase |
GABAT |
Mouse brain GABA-T full length |
Rabbit |
Jang-Yen Wu gift |
1:1000 |
|
| Cytochrome oxidase subunit IV |
COX IV |
Human full length subunit IV |
Mouse |
Invitrogen |
1:500 |
9 |
| High-energy phosphate transferring kinases |
|
|
| Nucleoside diphosphate kinase A |
NDPK-A |
Human Synthetic Peptide region on N-terminus |
Rabbit |
Abbiotec |
1:500 |
|
| Adenylate kinase 1 |
AK1 |
Recombinant AK1 AA 1-194 |
Mouse |
Abnova |
1:500 |
|
| Adenylate kinase 2 |
AK2 |
Human Synthetic Peptide AA 1-100 |
Rabbit |
Abcam |
1:500 |
96 |
| Creatine kinase - BB-brain type |
CK-B |
Human full length-brain cells |
Rabbit |
Abcam |
1:500 |
|
| Creatine kinase - MM-Muscle type |
CK-M |
Human synthetic peptide |
Rabbit |
Bioss |
1:500 |
|
| Mitochondrial creatine kinase |
CK-mi |
Human peptide of CK-mi (uMtCK) |
Goat |
Santa Cruz |
1:500 |
|
| Cell and organelle specific markers |
|
|
|
| Cone M-Opsin |
M-opsin |
Mouse M-opsin peptide (residues 3-16) |
Rabbit |
Cheryl Craft gift |
1:1000 |
98 |
| Cone S-Opsin |
S-opsin |
Recombinant human blue opsin |
Rabbit |
Chemicon |
1:1000 |
97, 98 |
| Middle wavelength-sensitive cone arrestin |
M-Car |
AA 369-381 of C-terminus |
Rabbit |
Cheryl Craft gift |
1:500 |
99 |
| Pan-plasma membrane calcium ATPase |
pan-PMCA |
Human erythrocyte calcium ATPase |
Mouse |
Affinity Bioreagents |
1:400 |
9 |
| Sarcoplasmic-endoplasmic reticulum calcium ATPase 3 |
SERCA3 |
Synthetic Peptide: V(29) T D A R E R Y G P N(39) |
Rabbit |
Affinity Bioreagents |
1:500 |
9 |
| CHX10 |
CHX10 |
Human CHX10 AA 1 to 131 of N terminus |
Sheep |
Exalpha |
1:500 |
102 |
| Protein kinase C-α |
PKCα |
Rat PKCα AA 659-672 |
Mouse |
Serotec |
1:1000 |
9 |
| Calbindin |
Calb |
Chicken gut D28-K |
Mouse |
Swant |
1:1000 |
|
| Choline acetyltransferase |
ChAT |
Porcine CHAT N-terminus |
Sheep |
Chemicon |
1:500 |
|
| Disabled-1 |
DAB1 |
Mouse DAB1 AA 400-555 |
Rabbit |
Chemicon |
1:500 |
101 |
| GABA |
GABA |
GABA Glutaraldehyde-BSA |
Rabbit |
Chemicon |
1:1000 |
100 |
| Vesicular glutamate transporter 1 |
VGLUT1 |
Synthetic peptide from rat VGLUT1 |
Guinea pig |
Chemicon |
1:1000 |
9 |
| Glutamate/Aspartate transporter |
GLAST |
Synthetic peptide from Rat GLAST C-terminus |
Guinea pig |
Chemicon |
1:1000 |
|
| Glutamine synthetase |
GS |
Sheep brain GS |
Mouse |
Sigma |
1:1000 |
|
Table 1 of
Rueda, Mol Vis 2016; 22:847-885.