Table 1 of Rueda, Mol Vis 2016; 22:847-885.

Table 1. Cell and compartmental specific primary antibodies.

Primary antigen Abbreviation Immunogen Host Source Dilution References
Hexokinase 1 HK1 Human HK1 Rabbit Cell Signaling 1:500 89
Hexokinase 2 HK2 Human AA 341 size16-20 AA Rabbit Cell Signaling 1:200 88-90
Phosphofructokinase L1 PFK-L1 Human Synthetic Pep AA 669-699 C-terminus Rabbit Abgent 1:200  
Pyruvate kinase M1 PK-M1 Human Synthetic Peptide AA 398-416 Rabbit Sigma 1:500 91
Pyruvate kinase M2 PK-M2 Human AA 380-440 Rabbit Abcam 1:500 92
Pyruvate kinase M2 PK-M2 Human PK-M2 full length Rabbit Cell Signaling 1:500 30, 55
Pan-lactate dehydrogenase pan-LDH Human LDH Rabbit Santa Cruz 1:500  
Lactate dehydrogenase 5 LDH5 Human LDH AA 203-232 C-terminus Rabbit Abgent 1:500  
TCA cycle and OXPHOS        
Oxoglutarate (α-ketoglutarate) dehydrogenase OGDH Human OGDH AA 705-1000 Rabbit Thermo Scientific 1:500  
Glutaminase GLS Human GLS AA 580-670 Mouse Abcam 1:500 93-95
Glutamate dehydrogenase 1 GDH 1 Recombinant GDH1 AA 1-265 Mouse Novus Biologicals 1:500  
GABA transaminase GABAT Mouse brain GABA-T full length Rabbit Jang-Yen Wu gift 1:1000  
Cytochrome oxidase subunit IV COX IV Human full length subunit IV Mouse Invitrogen 1:500 9
High-energy phosphate transferring kinases    
Nucleoside diphosphate kinase A NDPK-A Human Synthetic Peptide region on N-terminus Rabbit Abbiotec 1:500  
Adenylate kinase 1 AK1 Recombinant AK1 AA 1-194 Mouse Abnova 1:500  
Adenylate kinase 2 AK2 Human Synthetic Peptide AA 1-100 Rabbit Abcam 1:500 96
Creatine kinase - BB-brain type CK-B Human full length-brain cells Rabbit Abcam 1:500  
Creatine kinase - MM-Muscle type CK-M Human synthetic peptide Rabbit Bioss 1:500  
Mitochondrial creatine kinase CK-mi Human peptide of CK-mi (uMtCK) Goat Santa Cruz 1:500  
Cell and organelle specific markers      
Cone M-Opsin M-opsin Mouse M-opsin peptide (residues 3-16) Rabbit Cheryl Craft gift 1:1000 98
Cone S-Opsin S-opsin Recombinant human blue opsin Rabbit Chemicon 1:1000 97, 98
Middle wavelength-sensitive cone arrestin M-Car AA 369-381 of C-terminus Rabbit Cheryl Craft gift 1:500 99
Pan-plasma membrane calcium ATPase pan-PMCA Human erythrocyte calcium ATPase Mouse Affinity Bioreagents 1:400 9
Sarcoplasmic-endoplasmic reticulum calcium ATPase 3 SERCA3 Synthetic Peptide: V(29) T D A R E R Y G P N(39) Rabbit Affinity Bioreagents 1:500 9
CHX10 CHX10 Human CHX10 AA 1 to 131 of N terminus Sheep Exalpha 1:500 102
Protein kinase C-α PKCα Rat PKCα AA 659-672 Mouse Serotec 1:1000 9
Calbindin Calb Chicken gut D28-K Mouse Swant 1:1000  
Choline acetyltransferase ChAT Porcine CHAT N-terminus Sheep Chemicon 1:500  
Disabled-1 DAB1 Mouse DAB1 AA 400-555 Rabbit Chemicon 1:500 101
GABA GABA GABA Glutaraldehyde-BSA Rabbit Chemicon 1:1000 100
Vesicular glutamate transporter 1 VGLUT1 Synthetic peptide from rat VGLUT1 Guinea pig Chemicon 1:1000 9
Glutamate/Aspartate transporter GLAST Synthetic peptide from Rat GLAST C-terminus Guinea pig Chemicon 1:1000  
Glutamine synthetase GS Sheep brain GS Mouse Sigma 1:1000