Figure 6. Fasudil inhibits the migration of LPS-stimulated RMG cells, as shown with the Transwell chamber migration assay. Transwell chamber migration of retinal microglial (RMG) cells treated with dimethyl sulfoxide (DMSO; solvent control), lipopolysaccharide (LPS; 1 μg/ml), fasudil (10, 25, or 50 μM) + LPS (1 μg/ml), or fasudil (50 μM) + SB203580 (20 mM) + LPS (1 μg/ml) for 12 h. Representative photomicrographs of the migration chamber membranes with attached microglial cells are shown. Values represent the means ± standard deviation (SD) of three independent experiments performed in triplicate. ^#p<0.05 compared with untreated cells, **p<0.05 compared with LPS-stimulated cells.