Figure 2. Fasudil does not affect the proliferation of RMG cells. Cell viability was measured with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. A: Cell viability following fasudil treatment in retinal microglial (RMG) cells. Cells were treated with concentrations ranging from 0 to 50 μM of fasudil for 24 h. B: RMG cells were treated with lipopolysaccharide (LPS; 1 μg/ml) in the presence and absence of fasudil at the concentration of 10, 25, and 50 μM for 24 h. According to the results, fasudil did not affect RMG cell viability. The results are expressed as mean ± standard deviation (SD) of three independent experiments.