Figure 2. Immunohistochemistry of selected molecules in scleral whole mounts. Immunohistochemistry in whole mounts of sclera for vimentin (red) in (A) B6 mouse control and (B) 3-day glaucoma sclera cells. C, D: Control (left) and 3-day glaucoma (right) labeling for α-SMA (B6 mice). E, F: Control (left) and 3-day glaucoma (right) labeling for cell adhesion molecule, α-actinin (red, CD1 mice). 4’,6-diamidino-2-phenylindole (DAPI) nuclear counterstain (blue). Scale bars=20 µm.