Figure 6. Immunohistochemical analysis of GFAP. A: Representative slice showing glial fibrillary acidic protein (GFAP) expression in healthy (n = 4) and ischemia/reperfusion (I/R)-injured retinas. Scale bar, 50 μm. B: Intensity index (GFAP) calculations revealed significantly lower GFAP levels in I/R-injured retinas following mutant (triple Y-F) self-complementary adenovirus-associated virus type 2 vector encoding brain-derived neurotrophic factor (tm-scAAV2- BDNF) treatment (n = 4) than in the no vehicle (n = 3; **p<0.01) retinas or the retinas treated with tm-scAAV2-GFP (n = 3; **p<0.01). Bars depict means ± standard deviation (SD).