Table 1 of Khan, Mol Vis 2016; 22:771-782.
Clone | Primer sequence (5’-3’) |
---|---|
pET21a-γS | F: GGGAGTTCCATATGTCTAAAACTGGAACC |
R: CCGGAATTCTTACTCCACAATGCG | |
pET21a-γSG57W | F: GAAAGGCCCAACTTTGCTTGGTACATGTACATCTTACCACAGGGAG |
R: GGTAAGATGTACATGTACCAAGCAAAGTTGGGCCTTTCATAAACAGCCC | |
T7 | F: TAATACGACTCACTATAGG |
T7 | R: TATGCTAGTTATTGCTCAG |
BGH | R: TAGAAGGCACAGTCGAGG |