Figure 6. Secretory function of RPE is impaired in Ins2^Akita mice. Pigment epithelium-derived factor (PEDF) and GLUT1 protein expression was assessed with western blot of RPE tissue from diabetic mice with 2, 4, 7, and 10 months of hyperglycemia (HG) and compared with age-matched wild-type (WT) controls. Quantitative data were obtained by normalization of β-actin bands and represents GLUT1 and PEDF expression in Ins2^Akita related to WT (control). n = 6. ns represents non-significant data determined with Sidak’s multiple comparisons test, p<0.05.