Figure 3. GLUT1 expression is stabilized in the cell membrane in response to hypoxia. GLUT1 protein levels in the soluble and membrane fraction of D407 cells cultured with 25 mM of D-glucose and subjected to normoxia and hypoxia, show a marked increase in GLUT1 expression in the membrane of cells cultured in hypoxic conditions. n = 3. *p<0.05 symbolizes a significant increase in GLUT1 expression in the membrane fraction of the cells cultured under hypoxia with high glucose concentration medium, determined with Sidak’s multiple comparisons test.