Figure 3. Single injection of RS1-expressing MSCs into the Rs1h mouse eye. A: Colocalization of RS1-expressing (red) and PDGFRα-expressing (green) constitutive mesenchymal stem cells (MSCs) and inducible
MSCs at 8 weeks post-injection in the inner nuclear layer (INL), showing a gradient of fluorescent staining in the inset (3X
higher magnification). GCL = ganglion cell layer; ONL = outer nuclear layer. B: Representative RS1 localization (red) and hematoxylin and eosin (H&E) staining of histology sections from the superior and
inferior central quadrants of the wild-type retina. IS = inner segments. C: H&E staining of histology sections from the superior and inferior central quadrants of the retina in the inducible MSC and
constitutive MSC-injected eyes compare to the untreated eye at 8 weeks post-injection. Scale bar = 50 µm.
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