Figure 1 of Simmons, Mol Vis 2016; 22:705-717.


Figure 1. Pou4f2Cre.A: Diagram of the Pou4f2 gene showing the exons, introns, and locations of the designed primers. B: Diagram showing the Pou4f2Cre construct and locations of the designed primers. The construct consisting of an internal ribosome entry site (IRES) Cre, fused to exon 1, and an inverted neomycin cassette. C: PCR product showing the DNA sequence unique to Pou4f2Cre, compared to the wild-type. These bands were sequenced to confirm the authenticity of the bands. D: PCR was used to determine the zygosity of Cre. Pou4f2+/+ mice were Pou4f2 positive and Cre negative. Pou4f2Cre/+ mice were Pou4f2 and Cre positive. Pou4f2Cre/Cre mice were Pou4f2 negative and Cre positive. E: Retina whole mounts immunostained for POU4F1 (BRN3A) and POU4F2 (BRN3B). F: Retinal cryosections immunostained for POU4F1 and POU4F2. The Pou4f2Cre/Cre retinas display a large reduction in POU4F1-positive cells and no POU4F2 immunoreactivity. G: 4,6-diamidino-2-phenylindole (DAPI) staining for the panel. F: No significant difference was found between the POU4F1- and POU4F2-positive cells across the Pou4f2Cre/+ and Pou4f2+/+ retinas (data not shown). Scale bar = 100 μm.