Table 1 of Uribe, Mol Vis 2016; 22:658-673.

Table 1. Primers used for RT–PCR in this work.

Gene Species Primer Sequences Exon PCR product size (bp)
POMGNT1 Hu, Mk, Rt, Mo Fw: 5‘-ATCCTACCACTTTGGCATCG-3′ 17 108
    Rv: 5‘-ACATTCCTGAGCTGGACACC-3′ 18  
  Bv Fw: 5‘-ATCCTACCACTTCGGCATCG-3′ 17 108
    Rv: 5‘-ACATTCCTGAGCTGGACACC-3′ 18  
POMT1 Hu, Mk Fw: 5‘-AATCATGTCCAGTGCCTTCC-3′ 9 165
    Rv: 5‘-ATCATGGGGTAGGTGTCCTG-3′ 10  
  Bv Fw: 5‘-AATCATGTCCAGTGCCTTCC-3′ 9 165
    Rv: 5‘-ATCATAGGGTAGGTGCTCTGGT-3′ 10  
  Rt, Mo Fw: 5‘-AATCATGTCCAGTGCCTTCC-3′ 9 165
    Rv: 5‘-ATCATGGGATAGGTGTTCTTGT-3′ 10  
POMT2 Hu, Mk Fw: 5‘-GGTTTGGTGGCTGAATCTGT-3′ 18 222
    Rv: 5‘-ATGGCTGGGAAGTAGTGGTG-3′ 19  
  Bv Fw: 5‘-TCCTGTGCCTCATAGTGCTG-3′ 7 238
    Rv: 5‘-TAGAAGTGCCTGTGCGAGTG-3′ 9  
  Rt Fw: 5‘-TCCTGTGCCTCATAGTGCTG-3′ 7 176
    Rv: 5‘-AGAGCCATACGCCAAGTGTT-3′ 8-9  
  Mo Fw: 5‘-GTCTGGTGGCTGAATCTGGT-3′ 18 221
    Rv: 5‘-ATGGCTGGGAAGTAGTGGTG-3′ 19  

The sequences of forward (Fw) and reverse (Rv) primers used in this work are indicated. The exon to which each primer aligns is indicated in the penultimate column. The PCR product size is indicated in the last column for each primer pair. Hu: human, Mk: monkey, Bv: bovine, Rt: rat, Mo: mouse. For the 661W cell line the mouse primers were used.

Uribe, Mol Vis 2016; 22:658-673. http://www.molvis.org/molvis/v22/658

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