Figure 4. Immunolocalization of POMGnT1 and protein O-mannosyltransferases POMT1 and POMT2 in 661W photoreceptors. Cells were immunostained with antibodies specific for POMT1 (A, green) and POMT2 (B, red). These two proteins colocalized nearly completely in the nuclei of the 661W cells (C, yellow). Double immunostaining with antibodies specific for POMGnT1 (green) and POMT1 (red) is shown in D and for POMGnT1 (green) and POMT2 (red) in G. Magnified views in E and H correspond to boxed areas in D and G, respectively, additionally stained with 4’,6-diamidino-2-phenylindole (DAPI) in blue in F and I. Significant colocalization between POMGnT1 and POMT1 (E, F) and between POMGnT1 and POMT2 (H, I) is observed. Each bar equals 20 μm, except in E, F, H, and I: 10 μm.