Figure 3. Immunolocalization of POMGnT1 in the 661W photoreceptors. Cells were doubly immunolabeled with antibodies specific to POMGnT1 (A, green) and to the Golgi marker GM130 (E, red). 4’,6-Diamidino-2-phenylindole (DAPI)-stained nuclei are shown in blue (B, C, D, H). D: A negative control without a primary antibody to POMGnT1 did not show any signal. F: POMGnT1 immunoreactivity was distributed between the nucleus and the cytoplasm in 661W cells and colocalized with GM130 in the cytoplasm (yellow). Magnified views in C, G and H correspond to boxed areas in B and F. Each bar equals 20 μm, except in C, G, and H: 5 μm.