Figure 2. Immunolocalization of POMGnT1 in the mouse neural retina. Double immunolabeling of mouse retinal sections for POMGnT1 (A, B, D, E, G; red), cone arrestin (cArr; D, I; green), or the specific Golgi marker, GM130 (F, G, green; I, red) was performed. POMGnT1 appeared immunoreactive in the IS layer of photoreceptors (A, E; asterisk). Nuclei stained with 4’,6-diamidino-2-phenylindole (DAPI) are displayed in blue (B–D, I). C: A negative control without a primary antibody did not show any signal in the IS layer. D: POMGnT1 was localized in the myoid portion of cones, where its immunostaining superimposes on cArr labeling (arrow). A portion of the IS layer from G is magnified in H, where POMGnT1 is shown to colocalize with GM130 within the myoid portion (yellow), where labeling of the latter is superimposed on cArr immunoreactivity (I; arrow). POMGnT1 red puncta in the IS in D and I not overlapped with cArr are presumed to belong to rod photoreceptors. Arrowheads point to non-specific immunostaining of retinal vessels with secondary antibodies to mouse immunoglobulin G in A, C, E. IS = inner segments; ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer; IPL = inner plexiform layer; GCL = ganglion cell layer; OS = outer segments, m = myoid; n = nucleus. Each bar equals 20 μm, except in D, H, and I: 5 μm.