Figure 2. Acute expression of inflammatory mediators in the ipsilateral eye after MCAO. Quantitative real-time PCR analysis shows a significant relative fold increase in interleukin-1 beta (IL-1β) (A) and tumor necrosis factor (TNF) (B) mRNA expression in the ipsilateral eye 8 h after stroke compared to sham surgery. At 24 h, expression of the inflammatory signaling genes monocyte chemoattractant protein-1 (CCL2) (C) and myeloperoxidase (MPO) (D) is increased following ischemia. For all experiments, n = 4/group. Error bars show mean ± standard error of mean (SEM). Abbreviations: GAPDH = glyceraldehyde 3-phosphate dehydrogenase, SH = sham, ST = stroke. *p<0.05; **p<0.01; ***p<0.001.