Figure 4. SAS, but not LM609, suppresses the expression of pro-angiogenic and pro-inflammatory genes in the RPE-EC co-culture system. RPE and endothelial cells were grown in co-cultures and separated using CD-31-coated magnetic beads as described in the Materials and methods section. Following separation, RPE cells were examined for gene expression using a PCR array including 88 genes encoding proteins involved in angiogenesis and inflammation. The mRNA levels are expressed relative to the mRNA levels observed in solo RPE cells (control; defined as 1). Results shown are mean ± SD.