Figure 5 of Parfitt, Mol Vis 2016; 22:518-527.

Figure 5. Pathway analysis of gene expression data generated from RNA sequencing. Pathway analysis shows that the biologic processes most affected by aging are cellular and metabolic processes. In young mice, genes involved with meibomian gland development and cellular defense were found to be statistically enriched whereas, in older mice, the number of pathways and genes involving differentiation, cell signaling, and cell regulation pathways were significantly altered.