Figure 4. Differential gene expression analysis of 3-month- and 2-year-old mice eyelids performed by CyberT. A total of 3,725 genes were considered differentially expressed by showing at least a twofold difference in the reads per length of transcript in kilobases per million mapped reads (RPKM) values and a Bonferroni corrected p value of less than 10⁻⁵ between the data sets. Seventy-seven genes were more highly expressed in male and female 3-month-old mice, whereas 418 genes were found to be expressed higher in the male and female 2-year-old tissue.