Figure 8. The presence of macrophages and microglia decreases after hNPC treatment. A: Wild-type Long Evans (LE^sham) have few macrophages and microglia, as detected with the Iba1 antibody. B: Retinal degenerate Royal College of Surgeons (RCS^sham) have Iba1+ cells throughout the retina. C: Similar to LE^sham, Royal College of Surgeons rats with a subretinal injection of human forebrain derived neural progenitor cells (RCS^hNPCs) have few Iba1+ cells. D: However, areas further from the injection site away from the graft (AFG) with less photoreceptor survival contain numerous macrophage and microglial cells. RGC = retinal ganglion cell layer, INL = inner nuclear layer, ONL = outer nuclear layer. Scale bar = 20 μm.