Figure 7. Validation of rescue gene expression using qRT-PCR analysis. Primers were designed for rescue genes with a fold change difference
(FCD) closest to 0. A: The top five retinal degenerate Royal College of Surgeons (RCSsham) versus wild-type Long Evans (LEsham) downregulated and Royal College of Surgeons rats with a subretinal injection of human forebrain derived neural progenitor
cells (RCShNPCs) versus RCSsham upregulated rescue genes. The top six RCSsham versus LEsham upregulated and RCShNPCs versus RCSsham downregulated rescue genes were examined. B: The photoreceptor-specific gene rhodopsin (Rho) was also probed.