Figure 7. Validation of rescue gene expression using qRT-PCR analysis. Primers were designed for rescue genes with a fold change difference (FCD) closest to 0. A: The top five retinal degenerate Royal College of Surgeons (RCS^sham) versus wild-type Long Evans (LE^sham) downregulated and Royal College of Surgeons rats with a subretinal injection of human forebrain derived neural progenitor cells (RCS^hNPCs) versus RCS^sham upregulated rescue genes. The top six RCS^sham versus LE^sham upregulated and RCS^hNPCs versus RCS^sham downregulated rescue genes were examined. B: The photoreceptor-specific gene rhodopsin (Rho) was also probed.