Appendix 2 of Kobayashi, Mol Vis 2016; 22:436-445.


Appendix 2.

To access the data, click or select the words “Appendix 2.” Tube formation assay on matrigel by human retinal endothelial cells (HRECs) in vascular smooth muscle cell-conditioned medium (VSMC-CM) with or without cyopreservation at -70° C. HRECs were cultured for 20 h in 96-well plates coated with a matrigel. The cells were cultured in VSMC-CM stimulated with or without IL-13 (2 ng/ml). The network formation with HRECs was quantified by the total numbers of master junctions, master segments, or meshes. VSMC-IL-13-conditioned medium with or without cryopreservation at -70° C has same effect. *p <0.05, **P <0.01. Bars are mean ± standard error of the mean (SEM, n = 4 per group).