Molecular Vision: Sundararajan, Mol Vis 2016; 22:401-423. Table 3

Table 3. In vitro study on Wistar rat lenses cultured for 24 h in Dulbecco’s modified Eagle’s medium: Activity of redox component system and Malondialdehyde concentration.

Groups of lenses (incubated in DMEM)*	Reduced glutathione (μmol/g wet weight of lens)	MDA (nmol/g wet weight of lens)
Group I	8.28±0.25 ^b,c,d,e,f,g	60.47±3.15 ^b,c,d,e,f,g
Group II	3.57±0.59 ^a,c,d,e,h	85.81±3.44 ^a,c,d,e,h
Group IIIa	6.27±0.63 ^{a,b,d,e,f,g,h}	71.99±2.86 ^{a,b,d,e,f,,g,h}
Group IIIb	5.73±0.27 ^{a,b,c,e,f,g,h}	76.63±3.81 ^{a,b,c,e,f,g,h}
Group IIIc	4.14±0.73 ^{a,b,c,d,f,g,h}	80.25±4.02 ^a,b,c,d,g,h
Group IIId	3.78±0.35 ^a,c,d,e,h	82.55±4.07 ^a,c,d,h
Group IIIe	3.69±0..26 ^a,c,d,e,h	83.89±5.03 ^a,c,d,e,h
Group IV	8.23±0.15 ^b,c,d,e,f,g	60.54±3.86 ^b,c,d,e,f,g

*Groups of lenses; Group I: Normal lenses incubated in Dulbecco’s modified Eagle’s medium (DMEM) alone (control); Group II: Lenses incubated in DMEM containing sodium selenite (100 μM selenite/ml of DMEM; selenite-challenged, untreated); GroupIIIa: Lenses incubated in DMEM and simultaneously exposed to sodium selenite (100 μM selenite/ml of DMEM) and chrysin (200 μM chrysin /ml of DMEM; selenite-challenged simultaneously chrysin-treated); Group IIIb: Lenses first incubated for 2 h in DMEM containing only chrysin (200μM chrysin/ml of DMEM) and then for up to 24 h in fresh DMEM containing only sodium selenite (100 μM selenite/ml of DMEM; 2 h chrysin pre-treatment, subsequent selenite-challenge); Group IIIc: Lenses first incubated for 30 min in DMEM containing only sodium selenite (100 μM selenite/ml of DMEM) and then for up to 24 h in fresh DMEM containing only chrysin (200μM chrysin/ml of DMEM; initial selenite-challenge [30 min], subsequent chrysin-treatment); Group IIId: Lenses first incubated for 1 h in DMEM containing only sodium selenite (100 μM selenite/ml of DMEM) and then for up to 24 h in fresh DMEM containing only chrysin (200 μM chrysin/ml of DMEM; initial selenite-challenge [1 h], subsequent chrysin- treatment); Group IIIe: Lenses first incubated for 2 h in DMEM containing only sodium selenite (100 μM selenite/ml of DMEM) and then for up to 24 h in fresh DMEM containing only chrysin (200μM chrysin/ml of DMEM; initial selenite-challenge [2 h], subsequent chrysin- treatment); Group IV: Lenses incubated in DMEM containing only chrysin (200μM chrysin/ml of DMEM). Statistical analysis: ^aStatistically significant difference (p<0.05) when compared with groups I values. ^bStatistically significant difference (p<0.05) when compared with group II values. ^cStatistically significant difference (p<0.05) when compared with group IIIa values. ^dStatistically significant difference (p<0.05) when compared with group IIIb values. ^eStatistically significant difference (p<0.05) when compared with group IIIc values. ^fStatistically significant difference (p<0.05) when compared with group IIId values. ^gStatistically significant difference (p<0.05) when compared with group IIIe values. ^hStatistically significant difference (p<0.05) when compared with group IV values. Abbreviation: DMEM - Dulbecco’s modified Eagle’s medium.