Table 1 of
Sundararajan, Mol Vis 2016; 22:401-423. Table 1 of
Sundararajan, Mol Vis 2016; 22:401-423.
Table 1. In vitro study on Wistar rat lenses cultured for 24 h in Dulbecco’s modified Eagle’s medium: Primer sequences and expected product sizes for the genes amplified.
| S. No. | Name of the gene | Primer sequence | Size of the amplicon |
|---|---|---|---|
| 1 | m-calpain | 5′ – ATCGCCTCCCTCACCTTGAAT– 3′ 5′ –AGCTTTCAACCTCCTCGGCTCC– 3′ | 411 bps |
| 2 | Lp82 | 5′ –GTGTTTCCGGCGGGCACCCA– 3′ 5′ –CGCCGCCAATAATAAAGCGCG– 3′ | 124 bps |
| 3 | EGR-1 | 5′ – CCTCCCATCACCTATACTGGCC – 3′ 5′ – GGGGTTCAGGCCACAAAGTG– 3′ | 76 bps |
| 4 | COX-1 | 5′ – TTCATCCGAGAAGTACTCATG – 3′ 5′ – CTGGTGGGTGAAGTGTTGTGC – 3′ | 306 bps |
| 5 | PMCA-1 | 5′ –TTGAGTGCTAATGGTGAGGCC– 3′ 5′–GGCATATGGTTCTCAGGCCTT– 3′ | 106 bps |
| 6 | NCX-1 | 5′–ATGGTCTACATGTTCCTCGGAG– 3′ 5′–CCCAGGGCCATCAAGGTCAGG– 3′ | 176 bps |
| 7 | SERCA-2 | 5′–GGTGAAGAAACGATTACAGCC– 3′ 5′–TTCTATTGCATTTTCAGCGTT– 3′ | 111 bps |
| 8 | Caspase-3 | 5′–GTTCATGTGTATGTTTTATAAG– 3′ 5′–GTCCATGGAATTTAACCTCT– 3′ | 432 bps |
| 9 | Caspase-8 | 5′ –GGATTTGAAATCTTTTAAGTT– 3′ 5′ –GGCGAGTCCCACATGTCCTGCA– 3′ | 297 bps |
| 10 | Caspase-9 | 5′ –GAGGTATTTTGGTAACCACG– 3 5′ –GTGGAGCATCCCATAAATGCA– 3 | 287 bps |
| 11 | GAPDH (internal control) | 5′- TCAAGAAGGTGGTGAAGCAGGC −3′ 5′- TTCAGTTACTCTCAAGAGCCA −3′ | 207 bps |