Figure 7. In vitro study on Wistar rat lenses cultured for 24 h in Dulbecco’s modified Eagle’s medium: Effect of chrysin on calpain activity. A: Mean lenticular activity of calpain. Groups of lenses; Group I: Normal lenses incubated in Dulbecco’s modified Eagle’s medium (DMEM) alone (control); Group II: Lenses incubated in DMEM that contained sodium selenite (100 μM selenite/ml of DMEM; selenite-challenged, untreated); Group IIIa: Lenses incubated in DMEM and simultaneously exposed to sodium selenite (100 μM selenite/ml of DMEM) and chrysin (200 μM chrysin/ml of DMEM; selenite-challenged simultaneously chrysin-treated). Statistical analysis was one-way ANOVA with post-hoc test (least significant difference). Values are expressed as the mean ± standard deviation (SD) of six determinations. ^aStatistically significant difference (p<0.05) when compared with the Group I and III values. ^bStatistically significant difference (p<0.05) when compared with the Group II values. B: Analysis of casein zymography. Groups of lenses; Group I: Normal lenses incubated in Dulbecco’s modified Eagle’s medium (DMEM) alone (control); Group II: Lenses incubated in DMEM that contained sodium selenite (100 μM selenite/ml of DMEM; selenite-challenged, untreated); Group IIIa: Lenses incubated in DMEM and simultaneously exposed to sodium selenite (100 μM selenite/ml of DMEM) and chrysin (200 μM chrysin/ml of DMEM; selenite-challenged simultaneously chrysin-treated). Statistical analysis of values derived with densitometric scanning of the casein zymography (values shown as a bar diagram to the right of the casein zymogram) and one-way ANOVA with the post-hoc test (least significant difference). Values are expressed as mean ± standard deviation (SD) of six determinations. ^aStatistically significant difference (p<0.05) when compared with the Group I and III values. ^bStatistically significant difference (p<0.05) when compared with the Group II values. Abbreviations: GA=glutamic acid; Lp82=lenticular preferred calpain 82.