Figure 3. The yield, purity, and viability of RGCs from different methods. A: The yield of retinal ganglion cells (RGCs) from IP is the highest, while that from flow cytometry (FC) is the lowest. B: The viability of two-step immunopanning–retinal ganglion cells (TIP-RGCs) was increased at the first 9 days with culture time and was much higher than that of the two-step immunopanning-magnetic–retinal ganglion cells (TIPM-RGCs). Although the activity of the TIP-RGCs began to decline slowly, they still survived for 20 days, which was far longer than the TIPM-RGCs. C: The purity of the RGCs isolated from TIP is around 81% and much lower than that from the TIPM method (about 95%).