Figure 4. Optic nerve inflammation negatively correlates with visual acuity and is more severe in Th17-EAE relative to Th1. A: Representative hematoxylin and eosin (H&E)-stained paraffin-embedded 5-µm optic nerve sections indicating criterion by which
all nerves were graded for inflammation severity on a scale of 0–3. B: Linear regression of average optokinetic reflex (OKT) values on optic nerve inflammation severity scores throughout the
study for the corresponding eye for Th1 (red circles, p = 0.0139) and Th17 (blue squares, p = 0.0055) at 8, 13, and 21 days
post-immunization (DPI) indicating significant negative correlations by Spearman correlation. n = 30 eyes (from 15 mice) per
group. C: Optic nerve inflammation severity scores for Th1 (red circles) and Th17 (blue squares) optic nerves indicating significantly
more inflammation in Th17 nerves harvested at only 21 DPI (n = 10 eyes from five mice, p = 0.0281) with an unpaired t test.