Figure 2. EAE visual deficits are asymmetric and episodic. A: Representative time course of optokinetic reflex (OKT) measurements (purple circles and green squares) and disease severity
scores (top, orange diamonds) for a transfer experimental autoimmune encephalomyelitis (EAE) mouse demonstrating the asymmetric
and episodic nature of visual deficits, the onset of which coincide with motor deficits. B, C: Means of daily acuity readings (cycles per degree) as measured by OKT for the more affected eyes (purple circles) and less
affected eyes (green squares) of the Th1 (B) and Th17 (C) transfer EAE mice. Error bars represent standard error of the mean (SEM). Number of eyes: n = 15 for 1–7 days post-immunization
(DPI), n = 10 for 8–12 DPI, and n = 5 for 13–21 DPI. D, E, F: Area under the curve (AUC; presented as % of maximum for each plot) for daily visual acuity values (cycles per degree) as
measured by OKT for each Th1 (left, red circles) and Th17 (right, blue squares) mouse for 0–7 DPI (n = 15; D), 0–12 DPI (n = 10; E), and 0–19 DPI (n = 5; F). Eyes from the same mouse are connected by a line. MA = more affected eye and LA = less affected eye for each mouse as determined
by the average OKT score for each eye. *p<0.05, **p<0.01, ***p<0.001 with one-tailed Wilcoxon matched-pairs test.