Table 1 of Zhou, Mol Vis 2016; 22:311-318.


Table 1. Primers, restriction enzymes and PCR conditions used to analyze the nitric oxide synthase (NOS)2 and NOS3 polymorphism.

Gene SNP ID Primer (5’-3’) Restriction enzyme Restriction fragment Tm (°C)
NOS2 rs4795067 F: TAATCCCAGCCAGAGAGA CG KspAI A:117 64
(iNOS) R: AGCCTGGCACATACTTGAAGGTTAA (MBI) G:23, 94bp
NOS3
(eNOS) rs1799983 F: CATGAGGCTCAGCCCCAGAA SduI T:206bp G:124, 82bp 60
R: AGTCAATCCCTTTGGTGCTCAC (MBI)
rs1800779 F: TCTGCCTCTCCCAGTCTCTCA BccI A:189bp 65
R: AGCACTCTCCAGGCACTTCAG (NEB) G:124,65bp