Figure 5. Xaliproden protects against the loss of tight junctions in the presence of TNF-α. ARPE-19 cells were seeded in 24 Transwell plates and grown in low serum medium for 4 weeks. The cells were then treated with tumor necrosis factor-α (TNF-α; 10 ng/ml), xaliproden (20 M), or a combination of the two or left untreated for 48 h before the transepithelial electrical resistance (TEER) was measured using a volt/ohm meter. Bars represent the average electrical resistance from triplicates ± standard deviation (SD). *, p = 0.005, as determined with one-way ANOVA. xal = xaliproden.