Figure 4. EEDK inhibits apoptosis in the cornea. A: Representative images of the cornea were stained with a terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling
(TUNEL) kit (Nuclei were stained with 4′,6-diamidino-2-phenylindole [DAPI]) and obtained with confocal microscopy (Original
magnification, 630X). An arrow indicates a TUNEL-positive cell (Apoptotic cells). Corneal cross-sections from non-treated
(control), benzalkonium chloride (BAC)-treated alone, 50 mg/kg ethanol extract of Diospyros kaki (EEDK)-treated, 10 mg/kg EEDK-treated, and positive control (Refresh Plus, preservative-free eye drops, Allergan)-treated
mice are shown. B: The quantification of TUNEL-positive cells. Scale bar = 50 μm. Experimental values are expressed as the mean ± standard
error of the mean (SEM) from three independent experiments (n = 8 mice per group, **p<0.01, ***p<0.001).