Figure 1. EEDK ameliorated dry eye severity. A: Corneal fluorescein staining. B: The fluorescein staining score. C: Tear break-up time (BUT). D: Tear volume with Schirmer’s test. E: Hematoxylin and eosin (H&E) staining were assessed in all five groups. The treatment groups were administered 50 mg/kg ethanol extract of Diospyros kaki (EEDK), 10 mg/kg EEDK, or normal saline. Refresh Plus (preservative-free eye drops, Allergan) was used as the positive control. Original magnification = 400X, scale bars = 50 μm. Experimental values are expressed as the mean ± standard error of the mean (SEM) from three independent experiments (n = 8 mice per group, **p<0.01, ***p<0.001).