Figure 4. Mouse Müller cell PER2::LUC rhythms depend on ongoing Per1 and Bmal1 expression. A: Bioluminescent rhythms recorded from the Müller cells cultured from PER2::LUC mice. B: PER2::LUC rhythms from the Müller cell cultures treated with control siRNA. C: PER2::LUC rhythms from the Müller cell cultures treated with the Lipofectamine transduction reagent. D: Loss of PER2::LUC rhythms from the Müller cell cultures treated with Per1 siRNA. E: Loss of PER2::LUC rhythms from the Müller cell cultures treated with Per2 siRNA. F: Loss of PER2::LUC rhythms from the Müller cell cultures treated with Bmal1 siRNA. Arrows indicate the introduction of the new culture medium.