Figure 5. NGF facilitates the proliferation of murine Müller cells via ERK1/2 and AKT signaling. Proliferation of Müller cells increased following nerve growth factor (NGF) treatment as analyzed with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. In addition, the TrkA receptor, extracellular signal-regulated kinases 1/2 (ERK1/2), or phosphatidylinositol 3-kinase (PI3K)/AKT inhibitor abolished the promotion of proliferation by NGF at the 24 h (A), 48 h (B), and 72 h (C) time points. n=6, * p<0.05, ** p<0.01, *** p<0.001.