Figure 5. The p.P49L mutation in NRL increased its ability to transactivate Rho expression. WT or p.P49L mutant NRL expression constructs were co-transfected into HEK293 cells with pGL3-Rho-promoter and pRL-TK plasmids. The empty p3X-FLAG-CMV-7.1 expression vector was used as a negative control. The luciferase activities of whole cell lysates were measured. Data are the average of three replicate experiments and error bars show +/−1.00 SD. The asterisk indicates a significant difference.