Figure 3. Localization of the mutant EPHA2-Myc proteins in Caco-2 cells. The ectopically expressed EPHA2-Myc protein (cyan) was detected in Caco-2 cells using an anti-Myc primary antibody and Cy5-conjugated secondary antibody. Nuclei (blue) were labeled with DAPI. Cells expressing the wild-type EPHA2-Myc (A) and p.P584L (B), p.A959T (E), or pV972fsX39 (F) mutant EPHA2-Myc show peripheral and some cytoplasmic localization of the protein. In the cells expressing p.T940I (C) or p.D942fsXC71 (D) mutant EPHA2-Myc protein, the protein is mostly seen in the perinuclear region (arrow). Mock transfected cells were used as a negative control (G). Representative images from two independent experiments are shown. Scale-bar=20 µm.