Table 4 of Dionne, Mol Vis 2016; 22:177-188.
|Limit of Detection||Lower limit of detection which is constant and set by the manufacturer.||Limit of detection is calculated for each slide as a function of background intensity.|
|Reliability||Quality Control checks, QC1 and QC2, provided by the manufacturer. However QC2 failed for 11/13 cytokines.||Each array includes material to assist in optimization.|
|Standard Deviation||Must load duplicates to determine standard deviation.||Standard deviation, n=4, accounted for within the array.|
|Standard Curve||Linearity was harder to achieve. All 13 cytokines had to be adjusted for outliers.||Only 19/40 cytokines required adjustment for outliers to achieve linearity.|
|Binding Affinity||Antibody affinity and magnetic interactions must be maintained.||Only antibody affinity must be maintained.|
|Sample Prep||Requires more sample manipulations.||Requires less manipulations but longer wash steps.|
|Ease of Use||User interface is straightforward. However, no optimization can be done to the scan.||Scanning can be optimized for more reliable results.|