Table 4 of
Dionne, Mol Vis 2016; 22:177-188. Table 4 of
Dionne, Mol Vis 2016; 22:177-188.
Table 4. Comparison of key factors between methods.
| Factors | Luminex | GenePix |
|---|---|---|
| Limit of Detection | Lower limit of detection which is constant and set by the manufacturer. | Limit of detection is calculated for each slide as a function of background intensity. |
| Reliability | Quality Control checks, QC1 and QC2, provided by the manufacturer. However QC2 failed for 11/13 cytokines. | Each array includes material to assist in optimization. |
| Standard Deviation | Must load duplicates to determine standard deviation. | Standard deviation, n=4, accounted for within the array. |
| Standard Curve | Linearity was harder to achieve. All 13 cytokines had to be adjusted for outliers. | Only 19/40 cytokines required adjustment for outliers to achieve linearity. |
| Binding Affinity | Antibody affinity and magnetic interactions must be maintained. | Only antibody affinity must be maintained. |
| Sample Prep | Requires more sample manipulations. | Requires less manipulations but longer wash steps. |
| Ease of Use | User interface is straightforward. However, no optimization can be done to the scan. | Scanning can be optimized for more reliable results. |