Figure 2. Differential expression of Pax6. Ten-micron horizontal cryosections of the eyelid were examined for Pax6 expression. The developing meibomian gland expressed cytoplasmic Pax6 as early as P4 in the elongating epithelial cord. At P7 and P11, expression was observed within the acinar region of the gland. Once the gland was fully developed (adult), Pax6 expression was significantly reduced within the acini. MG = meibomian gland; HF = hair follicle; EP = epidermis.