Figure 5. Nrf2-deficient mice exhibit more severe RGC death in central and medial retinas. A: Healthy, non-experimental autoimmune encephalomyelitis (EAE) retinal ganglion cell (RGC) counts establishing that 11-week-old, unchallenged wild-type (WT) and Nrf2 knockout (KO) mice do not differ in number of RGCs. Error bars represent standard deviation. n = 12 eyes from six WT mice, and n = 6 eyes from three KO mice. Unpaired two-tailed t test was used for statistical analysis. B: Average total regional RGCs counted in both eyes of each EAE mouse at 21 days post-immunization (DPI) for WT (blue left bars), nuclear factor-E2-related factor (Nrf2) KO (red middle bars), and healthy, age-, and strain-matched control mice (green right bars). n = 18–21 eyes from nine to 11 mice per group. Error bars represent standard error of the mean (SEM). **p<0.005, ***p<0.001, and ****p<0.0001 with the linear mixed-effects model. C: Linear regression analysis between the total RGCs and the corresponding area under the curve (AUC) of visual acuity as measured with optokinetic tracking (OKT) throughout the study.