Appendix 1 of Larabee, Mol Vis 2016; 22:1503-1513.

Appendix 1: Quantitation method for cell type-specific antibodies in optic nerves.

Representative photomicrographs of optic nerves from healthy non-EAE control, WT EAE, and Nrf2 KO EAE mice. CNPase (myelin marker) and Iba1 (macrophage/microglia marker) signal were quantified as % area positive for staining normalized to the area of optic nerve measured. Cells positive for CD3 (T-cell marker) and B220 (B-cell marker) were counted and normalized to area of optic nerve in the picture. Each data point represents the average signal from a medial and lateral section of optic nerve, each with 3 representative fields measured (6 total fields quantified per point). To access the table, click or select the words “Appendix 1.”