Figure 6. Effect of CDECM on the NF-κB activation in hConECs and hPECs. A: The cell lysates were immunostained for nuclear factor-kappa B p65 (NF-κB p65), nuclear factor of kappa light polypeptide
gene enhancer in B-cells inhibitor, alpha (IκBα), and phospho-IκBα (Ser32/36). The membranes were photographed with the Fusion
FX image acquisition system. The graphs show the densitometry quantification of pIκBα (B, n = 5), IκBα (C, n = 5), and NF-κB p65 (D, n = 6). β-actin and Lamin B were used as the controls for the whole cell fraction and the nucleus fraction, respectively.
Values are mean ± standard deviation (SD). Statistical significance is indicated as **p<0.01 and ***p<0.001.