Table 2 of Chaney, Mol Vis 2016; 22:1468-1489.
Primary Antigen | Imunogen | Source | Catalog. No. | Target | Host | Dilution |
---|---|---|---|---|---|---|
CHX10 | Recombinant amino acids 1-131 N-terminal of human protein | Exalpha Biologicals | X1180P | RPCs in development Bipolar cells in adult | Sheep | 1:200 |
CcnD1 | Recombinant fusion protein from mouse | Santa Cruz | SC-450 | Proliferative RPCs | Mouse | 1:200 |
PCNA | Purified PCNA | Chemicon | MA4076 | Proliferative RPCs | Mouse | 1:100 |
PKCα | Protein kinase C alpha | AbD Serotec | MCA1572 | Rod bipolars | mouse | 1:100 |
PMCA | Purified human erythrocyte ATPas | Affinity Bioreagents | MA3-914 | OPL, IPL some amacrine and bipolar cells | Mouse | 1:500 |
Recoverin | KLH-conjugated linear peptide corresponding to a sequence from the EF-hand 4 domain of human recoverin | Chemicon | AB5585 | Photoreceptors | Rabbit | 1:1500 |
Rhodopsin | Bovine rhodopsin C-terminus, last 9 amino acids | Chemicon | MAB5356 | Rod photoreceptors | Mouse | 1:5000 |
Secretagogin | Amino acids 186-276 C-Terminus of SCGN of human origin | Santa Cruz | SC-135395 | Cone bipolars | Rabbit | 1:1000 |
SV2B | amino acids 1-126 mapping at the rat N-terminust | Gift from Roger Janz | Photoreceptor and bipolar cell ribbon synapses | Rabbit | 1:1000 | |
TRPM1 | Synthetic peptide of transient potential cation, subfamily M, member 1 variant delta | Osenses | OST00037W | Dendrites of ON bipolar cells | Sheep | 1:300 |
VGluT1 | Synthetic peptide from rat VGLUT1 protein | Chemicon | AB5905 | Synaptic layers | Guinea Pig | 1:1000 |