Figure 5 of Chaney, Mol Vis 2016; 22:1468-1489.


Figure 5. GLE did not alter GS protein or gene expression in developing retinas. The developing and adult retinas from (A–E) control and (F–J) GLE mice were labeled with an antibody against GS. GS exhibited an age-dependent increase in expression from PN3 to PN60. A and F: At PN 3, only the proximal retina was labeled. B and G: By PN5, the entire retina was immunolabeled. C, D, H, and I: PN7 and PN10 images show that the GS-IR labeling pattern became more organized as the retina developed. E and J: The intensity of GS-IR Müller glial cells was not different in PN60 control and GLE retinas, as previously described [21]. The number of cyclin D3-IR Müller glial cells in the PN60 control and GLE retinas was not different [21]. Scale bar = 40 μm. K: In the control and GLE retinas, GS gene expression (Glul) was low from E16.5 to PN10 and then increased at PN10. There were no significant differences at any age. The mean ± SEM values represent the triplicate samples from four to five animals per treatment group per age. GLE = Gestational lead exposure; GS = glutamine synthetase; PN = postnatal; E = embryonic; SEM = standard error of the mean.