Figure 3 of Blixt, Mol Vis 2016; 22:1405-1420.


Figure 3. Assessment of the Pax6, Otx2, and RXRγ regulatory sequences in combination with the piggyBac integration system. A: An evolutionarily conserved sequence (indicated by the arrow) from the chicken PAX6gene, positioned upstream of the transcription start [44]. Color-coded peaks correspond to sequences that are highly conserved between the species. The protein coding exons are blue, the conserved intronic regions are salmon, the 5′ untranslated regions (UTRs) are yellow, and the conserved upstream (intergenic) regions are red. The numbers underneath denote distance in base pairs. BD: Diagrams of constructs and fluorescence micrographs of sectioned retinas electroporated at stage 25 with the Pax6.300, Otx2.294, or RXRγ208 driven Cre-LoxP piggyBac constructs and analyzed at stage 34. St = stage, ONL = outer nuclear layer, INL = inner nuclear layer, GCL = ganglion cell layer.