Figure 8. Primary hfRPE cells in SFM. A: Cell survival percentage of human fetal RPE (hfRPE) cells in serum-free medium (SFM) over several days with standard deviation
error bars compared with day 0 (100%). B: Gene expression ratios of serum-starved hfRPE cultures compared to day 0 serum-supplemented hfRPE cultures measured with
quantitative PCR (qPCR) for selected genes. Points to the left of 0 indicate decreased expression as a percentage compared
to day 0; points to the right indicate increased expression. Relative quantity (RQ) values for triplicate assays were calculated
using the –ΔΔCt method and normalized using the mean value of three reference transcripts. Percent change=(RQ–1) × 100.